Activation of softwood Kraft pulp at high solids content by endoglucanase and lytic polysaccharide monooxygenase

• Softwood Kraft pulp was successfully activated by mechano-enzymatic treatments. The activation endoglucanase TrCel45A surpassed that LPMO TrAA9A. Dissolution time decreased with increasing fines, fibrillation and porosity. combination of TrAA9A halved the original dissolution time. manufacturing man-made cellulose fibers starts wood fibers. Pulps can dissolve at different rates leave amounts undissolved particles. Thus, their properties be modified to achieve better dissolution. Enzymatic treatments are an effective means enhancing dissolution, this study compares effect (TrCel45A) lytic polysaccharide monooxygenase (LPMO, TrAA9A) on bleached softwood 20 % solids content. enzymes were applied individually in combination. Both increased fibrillation, fines content, porosity, water retention value, crystallinity index crystallite size, but largest changes achieved enzyme mixture. For example, fiber saturation point value 64 37 TrCel45A, 27 25 TrAA9A, 73 52 both Pulp reactivity indirectly assessed measuring cupriethylenediamine. average reference measured 642 s, while those pulps treated mixture 399, 473 298 respectively. decrease correlated increase value.